Sampdoria are usually tough to beat at home and should progress.
Cagliari don't have much success outside of Sardinia and this is a tough draw for them.
Both of these sides had convincing wins in the earlier round of the competition.
Bet365 make Sampdoria 13/20 favourites for this one with Cagliari priced at 9/2.
Sampdoria can usually be relied upon at home. They are a fairly safe bet when they acts as hosts and have already seen off Inter, Genoa and Sassuolo here recently.
Their away form needs work and they almost tripped up again on the road against bottom club Crotone at the weekend, but fought back to snatch a point.
Their comeback frustrated the Crotone boss so much that he punched a wall and fractured his hand.
Bassano came here and were soundly beaten in the previous round of the competition. It finished 3-0 that day, but Cagliari should provide stiffer opposition.
Overall, the hosts have lost just one of their last eight games, a thumping 4-1 defeat at the Juventus Stadium, but they are in good spirits and becoming hard to overcome.
Cagliari aren't great travellers, which doesn't bode well for this fixture. They have lost their two visits here and haven't been victorious since 2012.
They may have won at the weekend, but their away form is dreadful and they slipped to a limp 1-0 defeat to Chievo during their last away outing.
Their only victory away from Sardinia this season came at the San Siro, but Inter were in a state of disarray and Frank De Boer was struggling to control his players.
Sampdoria have home advantage and will surely emerge victorious from this fixture. Back them at 13/20 with Bet365
There aren't many goal fests when these sides meet, with just 7 goals in the last 5 meetings at this stadium. Back Under 2.5 Goals at Evens.
Back Fabio Quagliarella (if he plays) to score anytime at 6/5 with Bet365